other:Article Title: Synapse-specific opioid modulation of thalamo-cortico-striatal circuits
Article Snippet: DOI: https://doi.org/10.7554/eLife.45146 14 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 6 GraphPad Software, algorithm Imaris Bitplane Software, algorithm Zen Zeiss Software, algorithm Chart 5 AD Instruments Software, algorithm Matlab r2007b and r2018a Mathworks Software, algorithm R (3.5.0) R Development Core Team Software, algorithm Ephus Vidrio Technologies, LLC Software, algorithm Custom data analysis software https://gitlab.com/ maolab/opi_ syn_circuit.git Data and analysis scripts related to quantification in manuscript and rebuttal All procedures were approved by Oregon Health and Science University Institutional Animal Care and Use Committee (IACUC) under protocol IP00000955, and Institutional Biosafety Committee under protocol IBC-10–40.
Article Title: Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent manner
Article Snippet: (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- Akt Pan, (rabbit monoclonal, clone C67E7) Cell signalling technology Ozyme Cat#: 4691; RRID:AB_915783 WB (1:1000) Antibody Anti- phospho- Akt, S473, (rabbit monoclonal, clone D9E) Cell signalling technology Ozyme Cat#: 4060; RRID:AB_2315049 WB (1:2000) Antibody Anti- mouse IgG (H + L) Secondary antibody HRP (Goat polyclonal) Thermo scientific Cat#: 31430; RRID:AB_228307 WB (1:5000) Antibody Anti- rabbit IgG(H + L) Secondary antibody HRP (Goat polyclonal) Thermo scientific Cat#: 31460; RRID:AB_228341 WB (1:10000) Chemical compound, drug vitamin C Sigma Aldrich Merck, Favre et al., 2011 A5960 Chemical compound, drug vitamin E Sigma Aldrich Merck, Favre et al., 2011 T3251 Chemical compound, drug Mito- tempo Sigma Aldrich Merck, Freed et al., 2014 SML0737 Chemical compound, drug catalase Sigma Aldrich Merck, Bouvet et al., 2007 C3155 Chemical compound, drug PEG- superoxide dismutase (SOD) Sigma Aldrich Merck, Bouvet et al., 2007 S9549 Chemical compound, drug Estetrol (E4) Sigma Aldrich Merck, Abot et al., 2014 SML1523 Chemical compound, drug ICI 182 780 Tocris Biotechne, Meyer et al., 2010 1047 Chemical compound, drug G- 1 ((±)–1-[(3aR*,4S*,9bS*)–4- (6- Bromo- 1,3- benzodioxol- 5- yl)–3 a,4,5,9b- tetrahydro- 3Hcyclopenta[c]quinolin- 8- yl)]- ethanone Cayman chemical Bertin Bioreagent, Meyer et al., 2010 10008933 chemical compound, drug G- 36 ((±)- (3aR*,4S*,9bS*)–4- (6- Bromo- 1,3- benzodioxol5- yl)–3 a,4,5,9b- tetrahydro- 8- (1- methylethyl))–3Hcyclopenta[c]quinoline Cayman chemical Bertin Bioreagent, Meyer et al., 2016 14,397 Sequence- based reagent N- (methylsulfonyl)–2- (2- propynyloxy)benzenehexanamide (MSPPOH) Cayman chemical Bertin Bioreagent, Dietrich et al., 2009 75,770 Chemical compound, drug Grammostola spatulata mechanotoxin 4 (GsMTx4) Alomone Labs, John et al., 2018 STG- 100 Chemical compound, drug YODA1 Bertin Bioreagent, Lhomme et al., 2019 SML1558 Chemical compound, drug ATPγS Tocris Biotechne, Kukulski et al., 2009 4080 Chemical compound, drug 4- hydroxy- 2,2,6,6- tetramethylpiperidine (TEMPOL) Sigma Aldrich Merck, Freidja et al., 2014 176,141 commercial assay or kit Nitric oxide metabolite detection kit Cayman Chemical 780,051 commercial assay or kit Hydrogen peroxide assay kit Abcam Ab102500 commercial assay or kit ATP determination kit Invitrogen Molecular Probes A22066 Continued
RNAscope:Article Title: Synapse-specific opioid modulation of thalamo-cortico-striatal circuits
Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (M. musculus, C57BL/6J) wildtype Jackson Laboratories Stock # 000664 RRID: IMSR_JAX: 000664 Continued on next page Birdsong et al. eLife 2019;8:e45146. .. DOI: https://doi.org/10.7554/eLife.45146 13 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug SR95531 Hello Bio Cat# HB0901 Chemical compound, drug Picrotoxin Hello Bio Cat# HB0506 Chemical compound, drug [Met5]-enkephalin Sigma Aldrich Cat# M6638 Chemical compound, drug DAMGO Sigma Aldrich Cat# E7384 Chemical compound, drug CTAP R and D Systems/Tocris Cat# 1560 Chemical compound, drug Naloxone Abcam Cat# ab120074 Chemical compound, drug DPDPE Sigma Aldrich Cat# E-3888 Chemical compound, drug Naltrindole Sigma Aldrich Cat# N-115 Chemical compound, drug ICI 174,864 R and D Systems/Tocris Cat# 0820 Chemical compound, drug MK801 Hello Bio Cat# HB0004 Chemical compound, drug CPP R and D Systems/Tocris Cat# 0173 Chemical compound, drug CGP 55845 R and D Systems/Tocris Cat# 1248 Chemical compound, drug Bestatin Sigma Aldrich Cat# B8385 Chemical compound, drug Thiorphan Sigma Aldrich Cat# T6031 Chemical compound, drug MPEP R and D Systems/Tocris Cat# 1212 Chemical compound, drug Red Retrobeads IX Lumafluor Inc Cat# R180 Chemical compound, drug Green Retrobeads IX Lumafluor Inc Cat# R180 Chemical compound, drug DNQX Sigma Aldrich Cat# D0540 Chemical compound, drug QX314 R and D Systems/Tocris Cat# 2313 Commercial assay, kit RNAscope Multiplex Fluorescent Assay Advanced Cell Diagnostics Cat# 320850 Commercial assay, kit RNAscope ProbeMm-Oprd1 Advanced Cell Diagnostics Cat# 427371 Commercial assay, kit RNAscope ProbeMm-Pvalb-C2 Advanced Cell Diagnostics Cat# 421931-C2 Software, algorithm FIJI 1.49b Wayne Rasband, NIH PMID: 22743772 Software, algorithm AxoGraph 1.4.4 Axograph Software, algorithm Microsoft Excel 2011 Microsoft Corp. Software, algorithm Illustrator CS5 Adobe Systems Software, algorithm Photoshop CS5 Adobe Systems Continued on next page Birdsong et al. eLife 2019;8:e45146. .. DOI: https://doi.org/10.7554/eLife.45146 14 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 6 GraphPad Software, algorithm Imaris Bitplane Software, algorithm Zen Zeiss Software, algorithm Chart 5 AD Instruments Software, algorithm Matlab r2007b and r2018a Mathworks Software, algorithm R (3.5.0) R Development Core Team Software, algorithm Ephus Vidrio Technologies, LLC Software, algorithm Custom data analysis software https://gitlab.com/ maolab/opi_ syn_circuit.git Data and analysis scripts related to quantification in manuscript and rebuttal All procedures were approved by Oregon Health and Science University Institutional Animal Care and Use Committee (IACUC) under protocol IP00000955, and Institutional Biosafety Committee under protocol IBC-10–40.
Multiplex Assay:Article Title: Synapse-specific opioid modulation of thalamo-cortico-striatal circuits
Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (M. musculus, C57BL/6J) wildtype Jackson Laboratories Stock # 000664 RRID: IMSR_JAX: 000664 Continued on next page Birdsong et al. eLife 2019;8:e45146. .. DOI: https://doi.org/10.7554/eLife.45146 13 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug SR95531 Hello Bio Cat# HB0901 Chemical compound, drug Picrotoxin Hello Bio Cat# HB0506 Chemical compound, drug [Met5]-enkephalin Sigma Aldrich Cat# M6638 Chemical compound, drug DAMGO Sigma Aldrich Cat# E7384 Chemical compound, drug CTAP R and D Systems/Tocris Cat# 1560 Chemical compound, drug Naloxone Abcam Cat# ab120074 Chemical compound, drug DPDPE Sigma Aldrich Cat# E-3888 Chemical compound, drug Naltrindole Sigma Aldrich Cat# N-115 Chemical compound, drug ICI 174,864 R and D Systems/Tocris Cat# 0820 Chemical compound, drug MK801 Hello Bio Cat# HB0004 Chemical compound, drug CPP R and D Systems/Tocris Cat# 0173 Chemical compound, drug CGP 55845 R and D Systems/Tocris Cat# 1248 Chemical compound, drug Bestatin Sigma Aldrich Cat# B8385 Chemical compound, drug Thiorphan Sigma Aldrich Cat# T6031 Chemical compound, drug MPEP R and D Systems/Tocris Cat# 1212 Chemical compound, drug Red Retrobeads IX Lumafluor Inc Cat# R180 Chemical compound, drug Green Retrobeads IX Lumafluor Inc Cat# R180 Chemical compound, drug DNQX Sigma Aldrich Cat# D0540 Chemical compound, drug QX314 R and D Systems/Tocris Cat# 2313 Commercial assay, kit RNAscope Multiplex Fluorescent Assay Advanced Cell Diagnostics Cat# 320850 Commercial assay, kit RNAscope ProbeMm-Oprd1 Advanced Cell Diagnostics Cat# 427371 Commercial assay, kit RNAscope ProbeMm-Pvalb-C2 Advanced Cell Diagnostics Cat# 421931-C2 Software, algorithm FIJI 1.49b Wayne Rasband, NIH PMID: 22743772 Software, algorithm AxoGraph 1.4.4 Axograph Software, algorithm Microsoft Excel 2011 Microsoft Corp. Software, algorithm Illustrator CS5 Adobe Systems Software, algorithm Photoshop CS5 Adobe Systems Continued on next page Birdsong et al. eLife 2019;8:e45146. .. DOI: https://doi.org/10.7554/eLife.45146 14 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 6 GraphPad Software, algorithm Imaris Bitplane Software, algorithm Zen Zeiss Software, algorithm Chart 5 AD Instruments Software, algorithm Matlab r2007b and r2018a Mathworks Software, algorithm R (3.5.0) R Development Core Team Software, algorithm Ephus Vidrio Technologies, LLC Software, algorithm Custom data analysis software https://gitlab.com/ maolab/opi_ syn_circuit.git Data and analysis scripts related to quantification in manuscript and rebuttal All procedures were approved by Oregon Health and Science University Institutional Animal Care and Use Committee (IACUC) under protocol IP00000955, and Institutional Biosafety Committee under protocol IBC-10–40.
Fluorescence:Article Title: Synapse-specific opioid modulation of thalamo-cortico-striatal circuits
Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (M. musculus, C57BL/6J) wildtype Jackson Laboratories Stock # 000664 RRID: IMSR_JAX: 000664 Continued on next page Birdsong et al. eLife 2019;8:e45146. .. DOI: https://doi.org/10.7554/eLife.45146 13 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug SR95531 Hello Bio Cat# HB0901 Chemical compound, drug Picrotoxin Hello Bio Cat# HB0506 Chemical compound, drug [Met5]-enkephalin Sigma Aldrich Cat# M6638 Chemical compound, drug DAMGO Sigma Aldrich Cat# E7384 Chemical compound, drug CTAP R and D Systems/Tocris Cat# 1560 Chemical compound, drug Naloxone Abcam Cat# ab120074 Chemical compound, drug DPDPE Sigma Aldrich Cat# E-3888 Chemical compound, drug Naltrindole Sigma Aldrich Cat# N-115 Chemical compound, drug ICI 174,864 R and D Systems/Tocris Cat# 0820 Chemical compound, drug MK801 Hello Bio Cat# HB0004 Chemical compound, drug CPP R and D Systems/Tocris Cat# 0173 Chemical compound, drug CGP 55845 R and D Systems/Tocris Cat# 1248 Chemical compound, drug Bestatin Sigma Aldrich Cat# B8385 Chemical compound, drug Thiorphan Sigma Aldrich Cat# T6031 Chemical compound, drug MPEP R and D Systems/Tocris Cat# 1212 Chemical compound, drug Red Retrobeads IX Lumafluor Inc Cat# R180 Chemical compound, drug Green Retrobeads IX Lumafluor Inc Cat# R180 Chemical compound, drug DNQX Sigma Aldrich Cat# D0540 Chemical compound, drug QX314 R and D Systems/Tocris Cat# 2313 Commercial assay, kit RNAscope Multiplex Fluorescent Assay Advanced Cell Diagnostics Cat# 320850 Commercial assay, kit RNAscope ProbeMm-Oprd1 Advanced Cell Diagnostics Cat# 427371 Commercial assay, kit RNAscope ProbeMm-Pvalb-C2 Advanced Cell Diagnostics Cat# 421931-C2 Software, algorithm FIJI 1.49b Wayne Rasband, NIH PMID: 22743772 Software, algorithm AxoGraph 1.4.4 Axograph Software, algorithm Microsoft Excel 2011 Microsoft Corp. Software, algorithm Illustrator CS5 Adobe Systems Software, algorithm Photoshop CS5 Adobe Systems Continued on next page Birdsong et al. eLife 2019;8:e45146. .. DOI: https://doi.org/10.7554/eLife.45146 14 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 6 GraphPad Software, algorithm Imaris Bitplane Software, algorithm Zen Zeiss Software, algorithm Chart 5 AD Instruments Software, algorithm Matlab r2007b and r2018a Mathworks Software, algorithm R (3.5.0) R Development Core Team Software, algorithm Ephus Vidrio Technologies, LLC Software, algorithm Custom data analysis software https://gitlab.com/ maolab/opi_ syn_circuit.git Data and analysis scripts related to quantification in manuscript and rebuttal All procedures were approved by Oregon Health and Science University Institutional Animal Care and Use Committee (IACUC) under protocol IP00000955, and Institutional Biosafety Committee under protocol IBC-10–40.
Software:Article Title: Synapse-specific opioid modulation of thalamo-cortico-striatal circuits
Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (M. musculus, C57BL/6J) wildtype Jackson Laboratories Stock # 000664 RRID: IMSR_JAX: 000664 Continued on next page Birdsong et al. eLife 2019;8:e45146. .. DOI: https://doi.org/10.7554/eLife.45146 13 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug SR95531 Hello Bio Cat# HB0901 Chemical compound, drug Picrotoxin Hello Bio Cat# HB0506 Chemical compound, drug [Met5]-enkephalin Sigma Aldrich Cat# M6638 Chemical compound, drug DAMGO Sigma Aldrich Cat# E7384 Chemical compound, drug CTAP R and D Systems/Tocris Cat# 1560 Chemical compound, drug Naloxone Abcam Cat# ab120074 Chemical compound, drug DPDPE Sigma Aldrich Cat# E-3888 Chemical compound, drug Naltrindole Sigma Aldrich Cat# N-115 Chemical compound, drug ICI 174,864 R and D Systems/Tocris Cat# 0820 Chemical compound, drug MK801 Hello Bio Cat# HB0004 Chemical compound, drug CPP R and D Systems/Tocris Cat# 0173 Chemical compound, drug CGP 55845 R and D Systems/Tocris Cat# 1248 Chemical compound, drug Bestatin Sigma Aldrich Cat# B8385 Chemical compound, drug Thiorphan Sigma Aldrich Cat# T6031 Chemical compound, drug MPEP R and D Systems/Tocris Cat# 1212 Chemical compound, drug Red Retrobeads IX Lumafluor Inc Cat# R180 Chemical compound, drug Green Retrobeads IX Lumafluor Inc Cat# R180 Chemical compound, drug DNQX Sigma Aldrich Cat# D0540 Chemical compound, drug QX314 R and D Systems/Tocris Cat# 2313 Commercial assay, kit RNAscope Multiplex Fluorescent Assay Advanced Cell Diagnostics Cat# 320850 Commercial assay, kit RNAscope ProbeMm-Oprd1 Advanced Cell Diagnostics Cat# 427371 Commercial assay, kit RNAscope ProbeMm-Pvalb-C2 Advanced Cell Diagnostics Cat# 421931-C2 Software, algorithm FIJI 1.49b Wayne Rasband, NIH PMID: 22743772 Software, algorithm AxoGraph 1.4.4 Axograph Software, algorithm Microsoft Excel 2011 Microsoft Corp. Software, algorithm Illustrator CS5 Adobe Systems Software, algorithm Photoshop CS5 Adobe Systems Continued on next page Birdsong et al. eLife 2019;8:e45146. .. DOI: https://doi.org/10.7554/eLife.45146 14 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Prism 6 GraphPad Software, algorithm Imaris Bitplane Software, algorithm Zen Zeiss Software, algorithm Chart 5 AD Instruments Software, algorithm Matlab r2007b and r2018a Mathworks Software, algorithm R (3.5.0) R Development Core Team Software, algorithm Ephus Vidrio Technologies, LLC Software, algorithm Custom data analysis software https://gitlab.com/ maolab/opi_ syn_circuit.git Data and analysis scripts related to quantification in manuscript and rebuttal All procedures were approved by Oregon Health and Science University Institutional Animal Care and Use Committee (IACUC) under protocol IP00000955, and Institutional Biosafety Committee under protocol IBC-10–40.
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